Aptagen, LLC
RNA
C-Reactive Protein (CRP)
Protein
125 nM (reported value)
SPR Binding Buffer: HEPES at pH 6.5 with 0.005% Tween 20
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Binding is stabilized with the addition of CaCl2 but the aptamer also produces signal in the absence of CaCl2
C-Reactive Protein (ID# 454)
5'rGprCprCprUprGprUprAprAprGprGprUprGprGprUprCprGprGprUprGprUprGprGprCprGprAprGprUprGprUprGprUprUprAprGprGprAprGprAprGprAprUprUprGprCp 3'
44
14346.65 g/mole
444700 L/(mole·cm)
56.82%
2.25
32.26
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
A Bini, M Mascini, et al. Development of an optical RNA-based aptasensor for C-reactive protein. Anal. Bioanal. Chem. 390(2008): 1077-1088
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