Aptamer Details

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A08 (Glyphosate Aptamer) (ID# 8583)

Aptamer Chemistry: DNA

Target: Glyphosate

Antigen/Target Category: Small Organic

Affinity (Kd): LOD: 0.6 µM (reported value)

Binding Conditions/Buffer: qPCR detection assay from magnetic bead preparation in tap water / deionized water. Previous work used 10 mM Tris-HCl.

Binding Temp: RT°C

Refolding Protocol: N/A

Specificity: Specific for glyphosate over 45 mM acetamiprid, 0.29 mM chlorpyrifos, 44 mM dimethoate, 39 mM trichlorfon, 0.62 mM methomyl, 48 mM propoxur, 6 mM glyphosate as separate solutions.

Sequence ():

Length: 84

Molecular Weight: 25921.7 g/mole

Extinction Coefficient: 781000 L/(mole*cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Shao Y, Tian R, Duan J, Wang M, Cao J, Cao Z, Li G, Jin F, Abd El-Aty AM, She Y. A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water. Sensors. 2023; 23(2):649. https://doi.org/10.3390/s23020649 Patent: CN105039347A

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