Aptamer Details

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PC12 Cells (Aptamer17a) (ID# 7618)

PC12 Cells
N/A nM (reported value)
Washed with selection buffer (5 mM MgCl in PBS) from and 1 mg/ml yeast tRNAfor 30 minutes.
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Binding conditions from Wang et al. (2003) and truncated aptamer 17 (Aptamer 17a) from Liu et al. (2011).

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Liu et al. "Dissection of the Functional Structure of Aptamer17, Which Specifically Recognizes Differential PC12 Cells." Nucleic Acid Therapeutics, 21(2011): 225-229.
Wang et al. "Single-stranded DNA Aptamers thatBbind Differentiated but not Parental Cells: Subtractive Systematic Evolution of Ligands by Exponential Enrichment." J. Biotechnol., 102(2003): 15-22.

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