Aptamer Details

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Avian Myeloblastosis Virus (a.1.1) (ID# 7574)

Aptamer Chemistry: RNA

Target: Avian Myeloblastosis Virus (AMV) RT

Antigen/Target Category: Protein

Affinity (Kd): 0.5 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (50 mM Tris-HC1 (pH 7.7), 200 mM potassium acetate, and 10 mM DTT)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 43

Molecular Weight: 13704.24

Extinction Coefficient: 408400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Chen and Gold. "Selection of High-Affinity RNA Ligands to Reverse Transcriptase: Inhibition of cDNA Synthesis and RNase H Activity." Journal of Biochemistry, 33(1994): 8746-8756.

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