Salmonella typhimurium (AptHis) Aptamer Details

Salmonella typhimurium (AptHis) (ID# 10013)

Aptamer Chemistry: DNA

Target: Salmonella typhimurium

Antigen/Target Category: Cells

Affinity (Kd): 1.52 µM (reported value)

Binding Conditions/Buffer: 1x AMP binding buffer (200 mM Tris-Cl, pH 8.8, 200 mM NaOH, and 1 mM MgCl2) equilibrated in 1 × phosphate buffered saline (PBS) (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4)

Binding Temp: Room temperature°C

Refolding Protocol: Incubated at 80°C for 5 min then incubated at room temperature for 10 min

Sequence ():

Length: 40

Molecular Weight: 12488 g/mole

Extinction Coefficient: 370000 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ji-Hyun Yeom, Boeun Lee, Daeyoung Kim, Jong-kook Lee, Suk Kim, Jeehyeon Bae, Yoonkyung Park, Kangseok Lee, Gold nanoparticle-DNA aptamer conjugate-assisted delivery of antimicrobial peptide effectively eliminates intracellular Salmonella enterica serovar Typhimurium, Biomaterials, Volume 104, 2016, Pages 43-51, ISSN 0142-9612, https://doi.org/10.1016/j.biomaterials.2016.07.009.

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