Aptagen, LLC
Bovine Catalase (CAT 1) (ID# 7692)
DNA
Bovine Catalase
Protein
237 nM (reported value)
CE Selection Buffer (1X TGK): 25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3).
CE Run Buffer: 3X TGK
CE Run Buffer: 3X TGK
N/A°C
Heated to 94° C for 10 min and then cooled at 0.5 ° C/s to 20° C.
At least a 100-fold decrease in affinity to lysozyme, trypsinogen, chymotrypsinogenA, and myoglobin relative to the target.
The Kd value listed was determined using affinity capillary electrophoresis. The Kd value using a fluorescence intensity assay was 430 nM.
5'-dCpdTpdTp dCpdTpdGp dCpdCpdCp dGpdCpdCp dTpdCpdCp dTpdTpdCp dCp dGpdApdCpdCpdTpdApdGp dCpdApdGpdTpdGpdGpdApdCpdAp dTpdGpdTpdGpdGpdCpdApdGpdGpdGpdTpdGp dApdApdGpdTpdGpdGpdCpdAp dTpdCpdGpdTpdCp dGpdGpdApdGpdApdCp dGpdApdGp dApdTpdApdGpdGpdCp dGpdGpdAp dCpdApdCp dTp-3'
82
25356.41
774800
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Ashley, J. et al. Selection of bovine catalase aptamers using non-selex. Electrophoresis 33(2012):2783-2789.
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