Exotoxin A (R14.33) Aptamer Details

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Exotoxin A (R14.33) (ID# 10029)

Aptamer Chemistry: DNA

Target: Exotoxin A

Antigen/Target Category: Protein

Affinity (Kd): 4.2-4.5 µM (reported value)

Binding Conditions/Buffer: 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride

Binding Temp: Room temperature°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specifically binds to Exotoxin A over bovine serum albumin (BSA) and Cholera toxin

Sequence ():

Length: 80

Molecular Weight: 24756 g/mole

Extinction Coefficient: 772800 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Hong KL, Yancey K, Battistella L, Williams RM, Hickey KM, Bostick CD, Gannett PM, Sooter LJ. Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human Serum. Biomed Res Int. 2015;2015:417641. doi: 10.1155/2015/417641.

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