Aptamer Details

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Fructose-bisboronic acid aptamer-sensor (ID# 8046)

Aptamer Chemistry: DNA

Target: Fructose

Antigen/Target Category: Small Organic

Affinity (Kd): 4 nM (reported value)

Binding Conditions/Buffer: Shinkai's receptor 50uM with 2% methanol.

Binding Temp: 24 °C°C

Refolding Protocol: Mix and incubate for 5 min at 95°C, and cool down at room temperature 24°C > 30 min

Specificity: N/A

Comments: sensor mixtures (fluorescein-labeled aptamer + competitor dabcyl-labeled oligonucleotide) to the addition of Shinkai’s receptor in the presence of increasing amounts of sugar, with the actual concentration of a receptor*complex at any particular glucose concentrations calculated from the Kd of Shinkai’s receptor.

Sequence ():

Length: 51

Molecular Weight: 15770.23

Extinction Coefficient: 523430

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yang KA, Barbu M, Halim M, et al. Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors. Nat Chem. 2014;6(11):1003-1008. doi:10.1038/nchem.2058

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