Aptagen, LLC
E. Coli Release Factor 1 (Clone II-1) (ID# 7528)
RNA
E. Coli Release Factor 1
Small Organic
30 nM (reported value)
Elution Buffer (250 mM imidazole)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Recombinant RF1 derived from e.coli (K-12 strain) was used to develop this aptamer.
5'-rGprGprAprCprCprGprAprGprAprAprGprUprUprAprCprCprCprUprGprUprAprAprUprCprUprUprAprGprGprAprUprGprAprAprUprCprGprCprAprUprGprCprUprCprUprAprGprCprGprAprCprCprUprUprUprUprCprGprGprCprUprUprCprGprGprCprGprUprAprCprGprCprAprCprAprUprCprGprCprAprGprCprAprAprCp-3'
85
27219.37
824600
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Sando, et al. "In vitro selection of RNA aptamer against Escherichia coli release factor 1." Bioorganic & Medicinal Chemistry Letters, 17(2007): 1216-1220.
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