Aptamer Details

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E. Coli Release Factor 1 (Clone II-1) (ID# 7528)

Aptamer Chemistry: RNA

Target: E. Coli Release Factor 1

Antigen/Target Category: Small Organic

Affinity (Kd): 30 nM (reported value)

Binding Conditions/Buffer: Elution Buffer (250 mM imidazole)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Recombinant RF1 derived from e.coli (K-12 strain) was used to develop this aptamer.

Sequence ():

Length: 85

Molecular Weight: 27219.37

Extinction Coefficient: 824600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Sando, et al. "In vitro selection of RNA aptamer against Escherichia coli release factor 1." Bioorganic & Medicinal Chemistry Letters, 17(2007): 1216-1220.

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