Aptagen, LLC
Nanobioprobe mediated DNA aptamer (ID# 7894)
DNA
Trinitrotoluene (TNT)
Other
100 nM (reported value)
10 mM Tris/HCl buffer, pH 7.6, 150 mM NaCl, 5 mM MgCl2
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The 5' end of the designed primer was labeled with FITC and thiol (-SHC6) to monitorthe progression of selection rounds and the binding of aptamers on a gold substrate respectively. The 3' end of the designed primer was labeled with biotin.
dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdGpdGpdGpdApdCpdApdGpdTpdCpdGpdApdTpdGpdGpdGpdApdCpdGpdGpdCpdApdApdApdCpdGpdGpdApdCpdCpdApdGpdTpdGpdTpdGpdTpdGpdGpdCpdGpdGpdTpdApdGpdApdTpdApdGpdTpdApdApdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp
88
27415.9
890400
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Priyanka, M. S., et al. "Nanobioprobe mediated DNA aptamers for explosive detection." Chem. Commun., 2014, 50, 1080 - 1082.
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