Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DE3A (ID# 8020)
2'-F-RNA
Sickle Hemoglobin
Protein
1.68 µM (reported value)
20 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2,
0.01% BSA
20°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
DE3A showed nearly identical bindingaffinities to FmetHbS and oxyHbS, with respective Kd valuesof 1.68 and 1.74 mM.
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGpfCpfCprGprApfUpfUprAprGprAprApfCpfUprGprGprGpfCpfUprGprAprGprGpfCprGpfUpfUpfCpfUprGpfCprApfUpfUpfUpfCprGprGpfUprGprApfUpfCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCprGprAprGprAp-3'
80
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Purvis, S. H., Keefer, J. R., Fortenberry, Y. M., Barron-Casella, E. A., & Casella, J. F. (2017). Identification of Aptamers That Bind to Sickle Hemoglobin and Inhibit Its Polymerization. Nucleic Acid Therapeutics, 27(6), 354–364. doi:10.1089/nat.2016.0646
Have your aptamer oligo synthesized ORDER
We are always looking for ways to improve. Please tell us what you think.