Aptamer Details

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SQ-2 (ID# 8066)

Aptamer Chemistry: RNA

Target: ALPPL-2

Antigen/Target Category: Protein

Affinity (Kd): 22.25-24.74 nM (reported value)

Binding Conditions/Buffer: 4.5 g/L glucose, 5 mmol/L MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL bovine serum albumin (BSA) in Dulbecco's PBS

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 76

Molecular Weight: 22995.6

Extinction Coefficient: 711400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Dua, P., Kang, H. S., Hong, S.-M., Tsao, M.-S., Kim, S., & Lee, D. -k. (2013). Alkaline Phosphatase ALPPL-2 Is a Novel Pancreatic Carcinoma-Associated Protein. Cancer Research, 73(6), 1934–1945. doi:10.1158/0008-5472.can-12-3682

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