Aptamer Details

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Tb-L8 (ID# 8062)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Protein

Affinity (Kd): 33 nM (reported value)

Binding Conditions/Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM MgCl2, 5 mM KCl, 1 mM,CaCl2, pH 7.4

Binding Temp: 20°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 44

Molecular Weight: 12601.1

Extinction Coefficient: 389600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Qiao, N., Li, J., Wu, X., Diao, D., Zhao, J., Li, J., … Lou, X. (2019). Speeding up in vitro Discovery of Structure-Switching Aptamers via Magnetic Cross-Linking Precipitation. Analytical Chemistry. doi:10.1021/acs.analchem.9b00081

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