Aptamer Details

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Lup an 1 allergen β-conglutin (SGQ) (ID# 7785)

Aptamer Chemistry: DNA

Target: β-conglutin

Antigen/Target Category: Protein

Affinity (Kd): 0.58 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 10 mM phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl₂, pH 7.4

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Showed no cross-reactivity with gliadin or γ-conglutin.

Comments: This is a truncated version of the clone 40 aptamer listed in the related aptamers section below. It is predicted to form a single G-quartet.

Sequence ():

Length: 11

Molecular Weight: 3509.33

Extinction Coefficient: 109700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

P Polo. Selection, characterisation and analytical application of DNA aptamer against the anaphylactic toxic allergen, β-conglutin, Lup an 1. (Doctoral Thesis). Retrieved from Tesis Doctorals en Xarxa. http://hdl.handle.net/10803/84036.

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