Aptagen, LLC
Aptamer 25 (ID# 7941)
DNA
Human norovirus (SMV-VLP)
Other
232 nM (reported value)
Phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20
23°C
Heat at 90°C for 5 min
The library was amplified using a fluorescein (FAM)-labeled forward constant region primer [5'-56-FAM/-AGTATACGTATTACCTGCAGC-3'] and a biotinylated reverse constant region primer [5'-/5Bios/-GCAAGATCTCCGAGATATCG-3']
dApdGpdTpdApdTpdApdCpdGpdTpdApdTpdTpdApdCpdCpdTpdGpdCpdApdGpdCpdCpdApdTpdCpdTpdGpdTpdGpdTpdGpdApdApdGpdApdCpdTpdApdTpdApdTpdGpdGpdCpdGpdCpdTpdCpdApdCpdApdTpdApdTpdTpdTpdCpdTpdTpdTpdCpdCpdGpdApdTpdApdTpdCpdTpdCpdGpdGpdApdGpdApdTpdCpdTpdTpdGpdCp
81
24864.21
770500
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Escudero-Abarca, B. I. et al. "Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains." PLOS One 2014: 1-11.
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