20 nM (reported value)
25 µM glucose 4 µM yeast tRNA 15 µM BSA 5 mM MgCl2 in PBS (pH = 7.4) 1 hour incubation with cells
heated at 95C for 5 min and cooled on ice for 10 min immediately
human astroglial cell line SVGp12 was used as a counter target
GMB128 only showed binding with cells at 4C while GMB131 showed binding at 4C as well as physiological temperature despite less affinity than GMB128
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kang et al. Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity (PLOSone)
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