Aptamer Details

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SARS-CoV-2 Variants’ Spike Protein DNA Aptamer M40 (ID# 8476)

Aptamer Chemistry: DNA

Target: Spike Protein of Wild Type SARS-CoV-2 Virus

Antigen/Target Category: Protein

Affinity (Kd): 155 pM (reported value)

Binding Conditions/Buffer: Binding Buffer containing 20 mM Hepes, 150 mM NaCl, 5 mM KCl, 2 mM MgCl2, 0.5 mM CaCl2, pH 7.4.

Binding Temp: 25°C

Refolding Protocol: 94 °C for 5 minutes and let to cool at room temperature for 20 minutes

Specificity: N/A

Comments: M40 showed strong binding affinity to various SARS-CoV-2 Virus including Wild-type, Alpha, Beta, Delta and Omicron with the Kd of 155pM, 280pM, 276pM, 187pM, and 165pM respectively. To be noted there is no specificity related tests in the article.

Sequence ():

Length: 51

Molecular Weight: 16.83 kDa

Extinction Coefficient: N/A

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Le TT, Benton DJ, Wrobel AG, Gamblin SJ. Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants. RSC Adv. 2023 May 19;13(22):15322-15326. doi: 10.1039/d3ra01382k. PMID: 37213341; PMCID: PMC10197177.

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