Aptagen, LLC
Interleukin 10 (IL-10) Receptor (R5A1) (ID# 7676)
RNA
Interleukin -10 receptor (IL-10)
Protein
12 nM (reported value)
150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82°C and slowly cooling to room temperature
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer was truncated and modified with 2'F and 2'O-methyl, but no KD's were given for these adaptations.
5'-rGprGprGprCprUprCprAprUprGprCprAprCprGprUprUprUprGprCprUprCprCprUprGprUprAprAprUprUprGprGprCprGprUprAprUprGprUprAprAprCprCprCprAprGprGprCprAprCprCprAprAprAprCprAprCprCprCprCprAprGprGprCprCprGprGprGprCprCprAprUprGprAprUprCprCprAprCprAprUprAp-3'
80
25585.42
765600
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Berezhnoy, A, et al. (2012). Isolation and optimization of murine il-10 receptor blocking oligonucleotide aptamers using high- throughput sequencing. The American Society of Gene & Cell Therapy
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