150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82°C and slowly cooling to room temperature
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer was truncated and modified with 2'F and 2'O-methyl, but no KD's were given for these adaptations.