Aptamer Details

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Ampicillin (AMP17) (ID# 7854)

Small Organic
13.4 nM (reported value)
Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl2.
Heated at 90°C for 3 min and then cooled at 4°C for 1 hour.
Did not bind to related antibiotics amoxicillin and benzylpenicillin, or unrelated antibiotics chloramphenicol, tetracycline, sulfadimethoxine, streptomycin, and kanamycin.
Because of AMP17's ability to aggregate on gold nanoparticles at lower salt concentrations than the other two aptamers, AMP17 was further evaluated in a colorimetric-fluorometric assay.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 402:2153-2161.

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