Streptococcus Pneumoniae (Lyd-3) Aptamer Details

Streptococcus Pneumoniae (Lyd-3) (ID# 10006)

Aptamer Chemistry: DNA

Target: Streptococcus Pneumoniae

Antigen/Target Category: Cells

Affinity (Kd): 661.8 ± 111.3 nM (reported value)

Binding Conditions/Buffer: 4.5 g/L glucose, 5mM MgCl2, 1mg/mL BSA in Dulbecco's PBS with CaCl2 and MgCl2 (Sigma D1283), pH 7.2

Binding Temp: 37°C

Refolding Protocol: Incubated at 95°C for 5 min, cooled on ice until incubation with bacteria, centrifuged at 3200 g for 5 min at 37 °C and washed 2 times using 1000 μL of binding buffer, After washing step solution was centrifuged again to pellet down the bacteria and supernatant was removed from the tube. Then, 300 μL of denaturated ssDNA library was incubated immediately with 1.0 × 108 CFU of bacteria pellet for 60 min using Tube Revolver/Rotator (Thermo, 88881001) at 15 rpm

Specificity: Specifically binds better to S. pneumoniae than S. pyogenes and E. faecalis

Sequence ():

Length: 80

Molecular Weight: 24238.7 g/mole

Extinction Coefficient: 734100 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Abdullah Tahir Bayraç, Sultan Ilayda Donmez, Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assay, Analytical Biochemistry, Volume 556, 2018, Pages 91-98, ISSN 0003-2697, https://doi.org/10.1016/j.ab.2018.06.024.

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