Aptamer Details

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C42 (ID# 8109)

Aptamer Chemistry: DNA

Target: Human PD1

Antigen/Target Category: Cells

Affinity (Kd): 23 nM (reported value)

Binding Conditions/Buffer: 5 ml of PBS-T containing 1 μg/ml bovine serum albumin (BSA) and 0.1 μg/ml tRNA

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 81

Molecular Weight: 25005.1

Extinction Coefficient: 772200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Khedri M, Abnous K, Rafatpanah H, Nabavinia MS, Taghdisi SM, Ramezani M. Development and Evaluation of Novel Aptamers Specific for Human PD1 Using Hybrid Systematic Evolution of Ligands by Exponential Enrichment Approach. Immunol Invest. 2020 Jul;49(5):535-554. doi: 10.1080/08820139.2020.1744639. Epub 2020 May 19. PMID: 32429721.

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