Aptagen, LLC
SP6 (ID# 8169)
DNA
SARS-Co V-2 Spike (RBD independent mechanism to neutralize SARS-CoV-2)
Protein
21 ± 4.6 nM (reported value)
PBS/3mM MgCl2/0.8 mg/mL BSA (PBS: 137 mM NaCl, 2.7 mM KCl, 10 nM NH4PO4, 1.76 mM KH2PO4 pH 7.4)
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Truncated version of Aptamer was showing higher binding affinity.
KD value of truncated version was not disclosed.
Sequence: 5'-dCpdCpdCpdApdTpdGpdGpdTpdApdGpdGpdTpdApdTpdTpdGpdCpdTpdTpdGpdGpdTpdApdGpdGpdGpdApdTpdApdGpdTpdGpdGpdGp-3'
5'-dGpdGpdGpdApdGpdApdGpdGpdApdGpdGpdGpdApdGpdApdTpdApdGpdApdTpdApdTpdCpdApdApdCpdCpdCpdApdTpdGpdGpdTpdApdGpdGpdTpdApdTpdTpdGpdCpdTpdTpdGpdGpdTpdApdGpdGpdGpdApdTpdApdGpdTpdGpdGpdGpdCpdTpdTpdGpdApdTpdGpdTpdTpdTpdCpdGpdTpdGpdGpdApdTpdGpdCpdCpdApdCpdApdGpdGpdApdCp-3’
86
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Schmitz, A., Weber, A., Bayin, M., Breuers, S., Fieberg, V., Famulok, M., & Mayer, G. (2021). A SARS‐CoV‐2 Spike Binding DNA Aptamer that Inhibits Pseudovirus Infection by an RBD‐Independent Mechanism. Angewandte Chemie International Edition, 60(18), 10279-10285.
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