Aptamer Details

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Interferon-γ (2’F-1) (ID# 7771)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Interferon-y/IFN-y/IFNG

Antigen/Target Category: Protein

Affinity (Kd): 4.4 nM (reported value)

Binding Conditions/Buffer: Binding Mixture: PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na₂HPO₄, and 1.1 mM KH₂PO₄, pH 7.4) containing 1 mM Mg²⁺ ions and 0.01% human serum albumin.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: This is a biphasic binding described by a two-Kd model, 4.4/716 nM.

Sequence ():

Length: 59

Molecular Weight: 18947.24

Extinction Coefficient: 566400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kubik, Mark F., et al. "Isolation and Characterization of 2ƒ??-Fluoro-, 2ƒ??-Amino-, and 2ƒ??-Fluoro-/Amino-Modified RNA Ligands to Human IFN-7 That Inhibit Receptor Binding." Journal of Immunology, 159 (1997): 259-267.

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