Aptagen, LLC
Tenascin-C (GBI-10) (ID# 7477)
DNA
Tenascin-C (TNC)
Protein
150 nM (reported value)
Binding Buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 0.1% (wt/vol) BSA, 0.1 % (vol/vol) Triton X-100, 0.1% (vol/vol) Tween 20)
4°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Selected using the TNC positive cell line U251.
At least a 10-fold decrease in Kd at 22° and 37°.
5'-dGpdGpdCpdTpdGpdTpdTpdGpdTpdGpdApdGpdCpdCpdTpdCpdCpdTpdCpdCpdCpdApdGpdApdGpdGpdGpdApdApdGpdApdCpdTpdTpdTpdApdGpdGpdTpdTpdCpdGpdGpdTpdTpdCpdApdCpdGpdTpdCpdCpdCpdGpdCpdTpdTpdApdTpdTpdCpdTpdTpdApdCpdTpdCpdCpdCp -3'
69
21127.69
626300
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Dion, Daniels, et al. "A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment." PNAS 100(2003): 15416-15421.
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