Aptamer Details

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Histone (H4K16Ac) (ID# 7722)

Aptamer Chemistry: DNA

Target: H4K16Ac

Antigen/Target Category: Peptide

Affinity (Kd): 47 nM (reported value)

Binding Conditions/Buffer: Washed with buffer A (150 mM NaCl, 5 mM Na₂HPO₄, 1 mM EDTA, pH 7.5) and PBS buffer (150 mM NaCl, 5 mM Na₂HPO₄. pH 7.5) three times.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 48

Molecular Weight: 14926.78

Extinction Coefficient: 476300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lin et al. "Recognition Imaging of Acetylated Chromatin Using a DNA Aptamer." Biophysical Journal, 97(2009): 1804-07.

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