Alpha-fetoprotein (AFP-specific) Aptamer Details

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Alpha-fetoprotein (AFP-specific) (ID# 10111)

Aptamer Chemistry: DNA

Target: Alpha-fetoprotein (AFP)

Antigen/Target Category: Protein

Affinity (Kd): 2.37 nM (reported value)

Binding Conditions/Buffer: 20 uL of 1% Bovine Serum Albumin (BSA) buffer

Binding Temp: 25 °C°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Competitive assay with free-floating Alpha-fetoprotein (AFP) and magnetic beads with surface-coated AFP.

Sequence ():

Length: 72 Base Pairs

Molecular Weight: 22471.5 g/mole

Extinction Coefficient: 691100 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Chao-Jyun Huang, Hsin-I. Lin, Shu-Chu Shiesh, Gwo-Bin Lee, An integrated microfluidic system for rapid screening of alpha-fetoprotein-specific aptamers, Biosensors and Bioelectronics, Volume 35, Issue 1, 2012, Pages 50-55, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2012.02.024.

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