Aptagen, LLC
Lectin concanavalin A (Con A-3-1m) (ID# 10082)
DNA
Lectin concanavalin A
Protein
Kd = 17nM (fluorescence particle-based assay) nM (reported value)
Incubated with Con A and FITC-PSA (counter-target, Kd = > 1 μM) in selection buffer (1xPBS, 2.5 mM MgCl2, 1mM CaCl2, 0.01% Tween 20)
Room Temperature°C
Not Reported
Counter Kd:> 1 μM (PSA). Also, ConA-3-1m showed virtually no binding to LcH, NPA, or VFA (other plant-derived mannose-binding lectins) at 10 nM
The actual aptamer crucially uses the modification 2-azidoethyl 2,3,4,6-tetra-O-acetyl-α-D-mannopyranoside at various points, which have been replaced with dTp's in the secondary structure shown (made with m-fold). This structure and the extinction coefficient may be inaccurate. The aptamer affinity was validated further through a BLI assay (Kd = 3.2 nM)
5'-dGpdApdTpdCpdCpdCpdApdGpdTpdCpdCpdGpdApdApdGpdTpdApdApdTpdCpdGpdTpdTpdGpdCpdApdTpdCpdTpdGpdCpdApdCpdGpdApdCpdTpdGpdGpdTpdGpdApdGpdCpdTpdTpdGpdApdGpdTpdGpdGpdCpdApdGpdApdApdGpdApdCpdTpdTpdGpdTpdGpdCpdApdApdApdCpdGpdGpdCpdTpdApdTpdApdGpdGp-3'
80
780200 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Chelsea K. L. Gordon, Diana Wu, Anusha Pusuluri, Trevor A. Feagin, Andrew T. Csordas, Michael S. Eisenstein, Craig J. Hawker, Jia Niu, Hyongsok Tom Soh; Click-Particle Display for Base-Modified Aptamer Discovery. ACS Chem. Biol. 20 December 2019; 14 (12): 2652–2662. https://doi.org/10.1021/acschembio.9b00587
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