Aptamer Details

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AMP18 (ID# 8232)

Aptamer Chemistry: DNA

Target: Ampcillin

Antigen/Target Category: Small Organic

Affinity (Kd): 9.8 nM (reported value)

Binding Conditions/Buffer: The ssDNA library (500 pmol) was pre-incubated for 3 min at 90 °C and immediately cooled for 1 h at 4 °C in 100 μL of binding buffer (20 mM Tris–HCl, 50 mM NaCl, 5 mM KCl, 5 mM MgCl2, pH 8.0) to stabilize the naturally occurring tertiary structure.

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Similar structured small antibiotics amoxicillin and benzylpenicillin do not bind to the aptamer.

Comments: Paper lists multiple aptamer sequences for binding the same molecule: AMP4 (21-mer 5′-CACGGCATGGTGGGCGTCGTG-3′) Kd-9.4nM AMP17 (19-mer 5′-GCGGGCGGTTGTATAGCGG-3′) Kd 13.4nM Multiple other references that cite this paper use the main sequence in this entry for their applications.

Sequence ():

Length: 19

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Song KM, Jeong E, Jeon W, Cho M, Ban C. Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 2012 Feb;402(6):2153-61. doi: 10.1007/s00216-011-5662-3. Epub 2012 Jan 7. PMID: 22222912. https://pubmed.ncbi.nlm.nih.gov/28917799/

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