Aptamer Details

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AMP18 (ID# 8232)

Small Organic
9.8 nM (reported value)
The ssDNA library (500 pmol) was pre-incubated for 3 min at 90 °C and immediately cooled for 1 h at 4 °C in 100 μL of binding buffer (20 mM Tris–HCl, 50 mM NaCl, 5 mM KCl, 5 mM MgCl2, pH 8.0) to stabilize the naturally occurring tertiary structure.
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Similar structured small antibiotics amoxicillin and benzylpenicillin do not bind to the aptamer.
Paper lists multiple aptamer sequences for binding the same molecule: AMP4 (21-mer 5′-CACGGCATGGTGGGCGTCGTG-3′) Kd-9.4nM AMP17 (19-mer 5′-GCGGGCGGTTGTATAGCGG-3′) Kd 13.4nM Multiple other references that cite this paper use the main sequence in this entry for their applications.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Song KM, Jeong E, Jeon W, Cho M, Ban C. Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 2012 Feb;402(6):2153-61. doi: 10.1007/s00216-011-5662-3. Epub 2012 Jan 7. PMID: 22222912. https://pubmed.ncbi.nlm.nih.gov/28917799/

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