Aptamer Details

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GNP DV2 (ID# 8072)

Aptamer Chemistry: DNA

Target: Dichlorvos (DV)

Antigen/Target Category: Small Organic

Affinity (Kd): 0.85 nM (reported value)

Binding Conditions/Buffer: 10 mM Tris, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl, pH 7.5

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 65

Molecular Weight: 20258.2

Extinction Coefficient: 655400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Chatterjee, B., Kalyani, N., Anand, A., Khan, E., Das, S., Bansal, V., … Sharma, T. K. (2020). GOLD SELEX: a novel SELEX approach for the development of high-affinity aptamers against small molecules without residual activity. Microchimica Acta, 187(11). doi:10.1007/s00604-020-04577-0

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