Aptagen, LLC
Nampt binding aptamer (NBA) (ID# 7775)
DNA
Nampt/Nicotinamide phosphoribosyltransferase
Protein
72.52 nM (reported value)
Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6.
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
No significant signal was observed from RBP4, Vaspin, adiponectin, human serum albumin, human IgG, and fibrinogen.
Limit of detection using ELAAS assay was 19 ng/ml. See SJ Lee et al (2012) for more details.
5'-dApdTpdAp dCpdCpdAp dGpdCpdTp dTpdApdTp dTpdCpdAp dApdTpdTp dGpdGpdGp dCpdApdGp dGpdApdCp dApdGpdGp dTpdGpdTp dCpdGpdGp dCpdTpdTp dGpdApdTp dApdGpdGp dCpdTpdGp dGpdGpdGp dTpdGpdTp dGpdTpdGp dTpdApdGp dApdTpdAp dGpdTpdAp dApdGpdTp dGpdCpdAp dApdTpdCp dTp-3'
76
23679.45
748600
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Park, J.-W., Tatavarty, R., Kim, D. W., Jung, H.-T., & Gu, M. B. (2012). Immobilization-free screening of aptamers assisted by graphene oxide. Chem. Commun., 48(15), 2071–2073. doi:10.1039/c2cc16473f SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248.
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