Aptamer Details

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hIL-10RA Aptamer (#411-J) (ID# 8005)

Aptamer Chemistry: RNA

Target: hIL-10RA

Antigen/Target Category: Protein

Affinity (Kd): 18 nM (reported value)

Binding Conditions/Buffer: 100 mM NaCl Selection Buffer (20mM, HEPES, 2mM CaCl2, 0.1mg/mL BSA)

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 84

Molecular Weight: 25252.9

Extinction Coefficient: 730200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Levay, Agata, et al. “ Identifying high-affinity aptamer ligands with defined cross-reactivity using high-throughput guided systematic evolution of ligands by exponential enrichment.” Nucleic Acids Research 12, (2015): 1-10.

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