Aptagen, LLC
AptHis-C (ID# 8132)
DNA
Hexahistidine
Small Organic
20.8 nM (reported value)
1% BSA, 0.1% Tween-20, 0.2 mg/mL of tRNA, DPBS, pH 7.4.
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5′-dTpdGpdGpdCpdApdApdGpdApdGpdGpdGpdTpdGpdTpdGpdCpdTpdTpdApdApdGpdGpdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGp-3′
39
12223.9
384000
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Wu, S.; Liu, Y.; Sun, H.; Zhong, M.; Dai, B.; Pan, B.; Shen, Z. An ssDNA aptamer specific for detection and purification of hexahistidine-tagged proteins. Anal. Biochem. 2020 Oct 15; 607: 113893. DOI: 10.1016/j.ab.2020.113893.
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