Aptamer Details

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Myo40-7-27 (ID# 7954)

Aptamer Chemistry: DNA

Target: Myoglobin

Antigen/Target Category: Protein

Affinity (Kd): 4.93 nM (reported value)

Binding Conditions/Buffer: 20 mM Hepes, 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1mM MgCl2, pH 7.3

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 40

Molecular Weight: 12131.88

Extinction Coefficient: 350000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, Q., et al. ƒ??Sensitive point-of-care monitoring of cardiac biomarker myoglobin using aptamer and ubiquitous person glucose meter.ƒ? Biosensors and Bioelecgtronics, 64 (2015): 161-164. Wang, Q., et al. ƒ??Screening of DNA Aptamers against Myoglobin Using a Positive and Negative Selection Units Integrated Microfluid Chip and its Biosensing Application.ƒ? Analytical Chemistry, 86 (2014): 6572-6579.

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