Aptamer Details

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Tobramycin (J6) (ID# 7458)

Aptamer Chemistry: RNA

Target: Tobramycin

Antigen/Target Category: Small Organic

Affinity (Kd): 2 nM (reported value)

Binding Conditions/Buffer: Selection Buffer: 140 mM NaCl, 5 mM KCl, 1 mM CaCl₂, 1mM MgCl₂, and 20 mM Tris Acetate (pH 7.4)

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 109

Molecular Weight: 35049.02

Extinction Coefficient: 1078300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, Yong, et al. "Specific binding of aminoglycoside antibiotics to RNA." Chemistry & Biology 2 (1995): 281-290

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