Aptamer binding took place in human blood sample produced by centrifuging 100 µL of whole human blood, collecting the pellet of red blood cells, resuspending the pellet in 50 µL of deionized water, and straining the resulting suspension.
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.