Aptamer Details

Back to Search Results

IR-A43 (ID# 8101)

Aptamer Chemistry: DNA

Target: recombinant insulin receptor extracellular domain

Antigen/Target Category: Protein

Affinity (Kd): 70 nM (reported value)

Binding Conditions/Buffer: 40 mM HEPES (pH 7.5), 102 mM NaCl, 5 mM KCl, 5 mM MgCl2, and 0.05% Tween-20

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Affinity value determined in the absence of insulin. Kd was determined to be 2.85 nM in the presence of 100 nM insulin.

dTs positions in sequence are 5-[N-(1-naphthylmethyl)carboxamide]-2’-deoxyuridine (Nap-dU).

Sequence ():

Length: 81

Molecular Weight: 22946.6

Extinction Coefficient: 711800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yunn NO, Park M, Park S, Lee J, Noh J, Shin E, Ryu SH. A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer. Nucleic Acids Res. 2021 Jan 25;49(2):700-712. doi: 10.1093/nar/gkaa1247. PMID: 33410883; PMCID: PMC7826266.

Have your aptamer oligo synthesized ORDER