Aptamer Details

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IR-A43 (ID# 8101)

recombinant insulin receptor extracellular domain
70 nM (reported value)
40 mM HEPES (pH 7.5), 102 mM NaCl, 5 mM KCl, 5 mM MgCl2, and 0.05% Tween-20
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Affinity value determined in the absence of insulin. Kd was determined to be 2.85 nM in the presence of 100 nM insulin.

dTs positions in sequence are 5-[N-(1-naphthylmethyl)carboxamide]-2’-deoxyuridine (Nap-dU).

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Yunn NO, Park M, Park S, Lee J, Noh J, Shin E, Ryu SH. A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer. Nucleic Acids Res. 2021 Jan 25;49(2):700-712. doi: 10.1093/nar/gkaa1247. PMID: 33410883; PMCID: PMC7826266.

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