Aptagen, LLC
Mycobacterium tuberculosis (strain H37Rv) (aptamer NK2) (ID# 7788)
DNA
Virulent mycobacterium tuberculosis (H37Rv)
Protein
31 nM (reported value)
Binding buffer: 25 mM Tris-HCl, 50 mM KCl, 200 mM NaCl, 0.2 mM EDTA, 5.0% (v/v) glycerol, and 0.5 mM DTT.
37°C
ssDNA was heated at 85°C for 15 min and snap-cooled on ice for 3 min.
Minimal binding observed against M. bovis Bacillus Guerin (BCG).
Inhibits H37Rv invasion of macrophage and stimulates secretion of cytokines IFN-&gamma, IL-15, and IL-17. Fan et al (2007) provided binding buffer while Fan et al (2012) provided sequence, Kd value, binding time, and other pertinent information.
5'-dGpdCpdGpdGpdGpdApdTpdCpdCpdTpdApdTpdGpdApdCpdGpdCpdApdTpdTpdGpdApdCpdCpdCpdApdCpdApdApdCpdApdCpdApdCpdTpdApdCpdTpdGpdTpdCpdGpdCpdTpdCpdGpdGpdTpdTpdCpdGpdApdApdCpdTpdTpdCpdGpdTpdGpdCpdGpdApdCpdTpdGpdTpdTpdCpdCpdCpdTpdApdTpdApdGpdTpdGpdApdGpdTpdCpdGpdTpdApdTpdTpdApdGpdApdApdTpdTpdCpdCpdGpdCp-3'
97
29764.31
909900
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Fan et al. "Aptamer from whole-bacterium SELEX as new theraputic reagent against virulent Mycobacterium Tuberculosis." Biochem. Bioph. Res. Co., 357, (2007): 743-748. Fan et al. "Aptamer Inhibits Mycobacterium Tuberculosis (H37Rv) Invasion of Macrophage." Mol. Biol. Rep., 39, (2012): 2157-2162.
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