Penicillin Binding Protein 2a (PBP2a) (Aptamer 1) Aptamer Details

Penicillin Binding Protein 2a (PBP2a) (Aptamer 1) (ID# 10009)

Aptamer Chemistry: DNA

Target: Penicillin-binding protein 2a (PBP2a)

Antigen/Target Category: Protein

Affinity (Kd): 82.97 ± 8.86 nM (reported value)

Binding Conditions/Buffer: 20 mmol/L HEPES buffer (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid) at pH 7.35, 120 mmol/L NaCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 5 mmol/L KCl

Binding Temp: 37°C

Refolding Protocol: Heated at 95°C for 5 min, then immediately placed into iced water, yeast tRNA was added to binding buffer to reduce background binding, libraries added to BSA-blocked blank wells and maintained at 37°C for 40 min

Specificity: Specific to MRSA over S. aureus, S. epidermidis, S. typhimurium, and E. coli

Sequence ():

Length: 78

Molecular Weight: 24229.7 g/mole

Extinction Coefficient: 767200 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ning Y, Wang X, Chen P, Liu S, Hu J, Xiao R, Li L, Lu F. Targeted inhibition of methicillin-resistant Staphylococcus aureus biofilm formation by a graphene oxide-loaded aptamer/berberine bifunctional complex. Drug Deliv. 2022 Dec;29(1):1675-1683. doi: 10.1080/10717544.2022.2079768.

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