Aptagen, LLC
9R-e1 (ID# 9645)
DNA
5-HMF
Small Organic
8.8 µM (reported value)
The 9th library of aptamers were incubated with the target for 1 gr at 37C, then an enzyme called T5 Exo was added to the mixture to digest unbound ssDNA library.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specific to 5-HMF, no other small molecules were compared though
dGpdTpdApdGpdCpdGpdGpdCpdGpdGpdTpdGpdTpdGpdGpdGpdCpdApdTpdTpdTpdTpdGpdGpdGpdCpdTpdApdApdAp
30
9374.1 g/mole
290400 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Liu, S., Cheng, J., Zhang, X., Qin, Y., Li, Y., Liu, H., Wu, Q., Hou, J., & Liu, X. (2025). T5 exonuclease improves the identification of aptamers specific for small molecules from the early screening library. Analytical Chemistry, 97(15), 8444–8451. https://doi.org/10.1021/acs.analchem.5c00251
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