1X PBS (pH 7.4)
Selections carried out in vivo in six- to eight-week-old female immunodeficient BALB/c mice (CAnN. Cg-Foxn1nu/Crl).
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
No binding to A431, A172, or Molm-13 cells - GD2-deficient cell lines.
The sequence for Clone B was identified, but that construct was not studied.