Aptamer Details

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Aptamer Chemistry: DNA

Target: Ampicillin

Antigen/Target Category: Small Organic

Affinity (Kd): 1.8 µM (reported value)

Binding Conditions/Buffer: SELEX Buffer: f 50 mM MES (pH 6.1), 500 mM NaCl, and 10 mM MgCl₂

Binding Temp: RT°C

Refolding Protocol: 95°C for 3 min, followed by gradual cooling to room temperature in SELEX buffer

Specificity: Specific to Ampicillin, other analogs tested include Aspirin, Kanamycin, Metronidazole, Penicillin G, 6-Aminopenicilanic Acid

Sequence ():

Length: 42

Molecular Weight: 12731.3 g/mole

Extinction Coefficient: 385200 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

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