Aptamer Details

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C3A WWP1 E3 ligase (ID# 8188)

Aptamer Chemistry: DNA

Target: C-lobe, HECT domain, WWP1 E3 ligase

Antigen/Target Category: Protein

Affinity (Kd): 1.9 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris/0.05% Tween-20/0 → 1.0 M NaCl (pH 7.3)

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Highly specific to the C-lobe of WWP1, a human HECT ubiquitin ligase.

Sequence ():

Length: 74

Molecular Weight: 22662.06

Extinction Coefficient: 700533

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Tucker, W., Kinghorn, A., Fraser, L., Cheung, Y.-W., & Tanner, J. (2018). Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1. International Journal of Molecular Sciences, 19(3), 763. doi:10.3390/ijms19030763

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