Aptamer Details

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PFOA Aptamer (ID# 8177)

Aptamer Chemistry: DNA

Target: Polyfluoroalkyl acid (Perfluorooctanoic acid, PFOA)

Antigen/Target Category: Small Organic

Affinity (Kd): 5.5 nM (reported value)

Binding Conditions/Buffer: pH 7.5 buffer (20 mM HEPES, 1 M NaCl, 10 mM MgCl2, and 5 mM KCl)

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 30

Molecular Weight: 9488.22

Extinction Coefficient: 308100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Park, J., Yang, K.-A., Choi, Y., & Choe, J. K. (2022). Novel ssdna aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water. Environment International, 158, 107000. https://doi.org/10.1016/j.envint.2021.107000

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