Aptamer Details

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NADH (ID# 7667)

Aptamer Chemistry: RNA

Target: NADH

Antigen/Target Category: Small Organic

Affinity (Kd): 37 nM (reported value)

Binding Conditions/Buffer: Selection Buffer (20 mM HEPES (pH 7.5), 300 mM KC1, and 5 mM MgC12) at room temperature.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 49

Molecular Weight: 15748.56

Extinction Coefficient: 474700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lauhon and Szostak. "RNA Aptamers That Bind Flavin and Nicotinamide Redox Cofactors." Journal of the American Chemical Society, 117(1994): 1246-1257.

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