Aptamer Details

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A2 (ID# 8125)

Aptamer Chemistry: DNA

Target: M0-like and M2-like macrophages and monocytes

Antigen/Target Category: Cells

Affinity (Kd): 20-45 nM (reported value)

Binding Conditions/Buffer: For individual aptamer binding, cells were incubated with aptamer that was in Wash Buffer supplemented with 10% FBS and 100 ug/mL tRNA.

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 52

Molecular Weight: 16149.5

Extinction Coefficient: 526500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Sylvestre, M.; Saxby, C.; Kacherovsky, N.; Gustafson, H.; Salipante, S.; Pun, S. Identification of a DNA Aptamer That Binds to Human Monocytes and Macrophages. Bioconjug. Chem. 2020 Aug 9; 31(8): 1899-1907. DOI: 10.1021/acs.bioconjchem.0c00247.

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