LipL32 DNA Aptamer LepDapt-5a (ID# 8409)
33.97 nM (reported value)
10 mM HEPES-KOH [pH 7.4], 150 mM NaCl
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Yeast tRNA was introduced in SELEX section as the competitor to DNA candidates.
LepDapt-5a was proven to be the most potent aptamer against LipL32 protein. It is a prolific diagnostic agent which can be used in developing potential diagnostic platforms with designing specific hybrid-heterodimeric aptamer to detect pathogenic Leptospira.