Room Temperature for 4 hours incubation with ion-selective-electrode (ISE) sensor
unspecific reaction and accumulation observed with erythrocyte, platelet and protein molecules
Best cell-sensitivity in buffer is -?25.21?mV/log10CA549. 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA) based covalent anchoring method is used to immobilize AS1411 onto the PGO decorated I- -ISE (PGO-I-ISE)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zhang R, Gu Y, Wang Z, Li Y, Fan Q, Jia Y. Aptamer cell sensor based on porous graphene oxide decorated ion-selective-electrode: Double sensing platform for cell and ion. Biosensors and Bioelectronics. 2018 Jun 15.
Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.