Aptamer Details

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OSM (ADR58) (ID# 7499)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Human Oncostatin M (OSM)

Antigen/Target Category: Protein

Affinity (Kd): 7 nM (reported value)

Binding Conditions/Buffer: Wash Buffer: 0.1% I-block, 0.05% Tween 20 in SCHMK buffer (110 mM NaCl, 1 mM MgCl₂, 20 mM HEPES (pH 7.0), 1 mM CaCl₂, 5 mM KCl)

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Can specifically antagonize OSM by binding to the gp130 receptor.

Sequence ():

Length: 70

Molecular Weight: 22572.51

Extinction Coefficient: 651800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Rhodes, Andrew, et al. "The generation and characterization of antagonist RNA aptamers to human oncostatin M." Journal of Biological Chemistry, 275 (2000): 28555-28561.

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