Aptamer Details

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Clon-3 (ID# 8193)

70.1 ± 14.2 nM nM (reported value)
Binding buffer: 50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES), 100 mM NaCl, 1 mM MgCl2, 5 mM KCl, and 1 mM CaCl2, pH = 7.5 Binding/Wash buffer (Capture SELEX): 10 mM Tris, 1 mM EDTA, 1 M NaCl, pH 7.4 Varying incubation times: 10min - 1hr
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Li, J., Ren, X., Zhao, J., & Lou, X. (2021). PD-L1 aptamer isolation via Modular-SELEX and its applications in cancer cell detection and tumor tissue section imaging. The Analyst, 146(9), 2910–2918. https://doi.org/10.1039/d1an00182e

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