Aptamer Details

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Anti Lung Carcinoma ( EJ4) (ID# 7871)

Lung Adenocarcinoma (H23)
60 nM (reported value)
0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes
denature by heating at 95C for 5 minutes, followed by rapid cooling on ice
HBE 135-E6/E7 (normal epithelial lung) cells used as counter targets
Reference did not list what primer sequences they used. The Author only mentioned that the primers were designed using IDTƒ??s oligo analyzer and met the following characteristics: a minimum hairpin structure, similar melting temperatures and minimal base pairing.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Jimenez et al. Generation of Lung Adenocarcinoma DNA Aptamers for Cancer Studies (PLOSone)

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